Hardness is caused by divalent metallic cations.
In addition, divalent metal adduction provided improved positive mode ionization efficiency for these peptides.
The rate of the coordination reaction between divalent metal ions and murexide in a water-in-oil microemulsion was determined by spectrophotometric technique to study the transport of the metal ions entrapped in the organogel formed by cationic surfactant cetyltrimethylammonium bromide.
