According to reports, ESC culture in vitro was more than two years and attained 450 serial passages. Embryonic germline ESC attained 70~80 passages at most.
Objective To explore germline mutation frequencies and features of mismatch repaired gene MSH6 in persons with different kinds of colorectal cancer.
Here, we show that removing the translational repressor Nanos from either GSCs or their precursors, the primordial germ cells (PGCs), causes both cell types to differentiate into germline cysts.
Often the result of a new germline or somatic mutation.
